Sudan stain - Sudan staining is the use of Sudan dyes to stain sudanophilic substances, usually lipids. Sudan lysochromes ( Sudan II, Sudan III, Sudan IV, Oil Red O, and Sudan Black B) are used. Sudan dyes have high affinity to fats, therefore they are used to demonstrate triglycerides, lipids, and..
Emulsion test - The emulsion test is a method to determine the presence of lipids using wet chemistry. The procedure is for the sample to be suspended in ethanol, allowing lipids present to dissolve. The ethanolic solution is then decanted into water. Since lipids do not dissolve in water, when the ethanol is..
Sudan IV - Sudan IV (C 24 H 20 N 4 O) is a lysochrome (fat-soluble dye) diazo dye used for the staining of lipids, triglycerides and lipoproteins on frozen paraffin sections. It has the appearance of reddish brown crystals with melting point 199 °C and maximum absorption at 520(357) nm. Sudan IV..
Sudan II - Sudan II (C 18 H 16 N 2 O) is a lysochrome (fat-soluble dye) azo dye used for staining of triglycerides in frozen sections, and some protein bound lipids and lipoproteins on paraffin sections. It has the appearance of red powder with melting point 156-158 °C and maximum absorption at..